Journal: World Journal of Gastroenterology

Article Title: Bile acids inhibit ferroptosis sensitivity through activating farnesoid X receptor in gastric cancer cells

doi: 10.3748/wjg.v30.i5.485

Figure Lengend Snippet: Bile acids enhanced proliferation and inhibited erastin-induced ferroptosis sensitivity in gastric cancer cells. A and B: Cell viability assay for HGC-27 and MKN-45 cells treated with three Bas; C and D: Cell viability assay for HGC-27 and MKN-45 cells treated with different concentration of BAs together with erastin (5 μM); E-H: Cell viability assay for two gastric cancer cell lines stimulated with erastin followed by chenodeoxycholic acid (50 μM) or control for 24 and 48 h; I and J: Malondialdehyde production in HGC-27 and MKN-45 cells; K: BODIPY-589/591 C11 staining to identify lipid reactive oxygen species in the cell lines under different treatments. Scale bar: 100 μm. a P < 0.05, b P < 0.01, c P < 0.001. These experiments were repeated three times. BAs: Bile acids; CA: Cholic acid; DCA: Dehydrocholic acid; CDCA: Chenodeoxycholic acid; MDA: Malondialdehyde; NS: Not significant.

Article Snippet: CA (S3742), dehydrocholic acid (DCA, S4562), CDCA (S1843), erastin (S7242), Ferrostatin-1 (Fer-1, S7243), and GW4064 (S2782) were purchased from Selleck Chemicals (Houston, TX, United States).

Techniques: Viability Assay, Concentration Assay, Control, Staining

Journal: World Journal of Gastroenterology

Article Title: Bile acids inhibit ferroptosis sensitivity through activating farnesoid X receptor in gastric cancer cells

doi: 10.3748/wjg.v30.i5.485

Figure Lengend Snippet: Bile acids inhibited ferroptosis sensitivity of gastric cancer cells by activating farnesoid X receptor. A and B: Cell viability of erastin-treated HGC-27 and MKN-45 cells with or without GW4064 treatment; C and D: HGC-27 and MKN-45 cells were transfected with shFXR or shNC plasmid. Successful construction was confirmed by western blot analysis; E and F: Cell viability assay of GC cells treated with different concentrations of erastin and CDCA (50 μM) transfected with shFXR or shNC for 24 h; G-I: Malondialdehyde (MDA) production and BODIPY-589/591 C11 staining of GC cells transfected with shFXR or shNC plasmid and treated with erastin together with or without CDCA for 24 h; J and K: GC cells were transfected with control or FXR-coding plasmid and confirmed through western blot analysis; L and M: Cell viability assay of GC cells treated with different concentrations of erastin and CDCA (50 μM) transfected with control or FXR-coding plasmid for 24 h.; N-P: MDA production and BODIPY-589/591 C11 staining of GC cells transfected with control or FXR-coding plasmid and treated with erastin together with or without CDCA for 24 h. Scale bar: 100 μm. a P < 0.05, b P < 0.01, c P < 0.001. These experiments were repeated three times. FXR: Farnesoid X receptor; NC: Negative control; CDCA: Chenodeoxycholic acid; MDA: Malondialdehyde; NS: Not significant.

Article Snippet: CA (S3742), dehydrocholic acid (DCA, S4562), CDCA (S1843), erastin (S7242), Ferrostatin-1 (Fer-1, S7243), and GW4064 (S2782) were purchased from Selleck Chemicals (Houston, TX, United States).

Techniques: Transfection, Plasmid Preparation, Western Blot, Viability Assay, Staining, Control, Negative Control

Journal: Molecular Carcinogenesis

Article Title: Interplay between bile acids and the gut microbiota promotes intestinal carcinogenesis

doi: 10.1002/mc.22999

Figure Lengend Snippet: Effects of cholic acid (CA) treatment on short‐chain fatty acid production and bile acid metabolism in Apc min/+ mice. A, Types of short‐chain fatty acids, including acetate, propionate, and butyrate, detected in the cecal contents. B, Types of bile acids detected in the feces, including CA, chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), lithocholic acid (LCA), α‐muricholic acid (α‐MCA), and β‐muricholic acid (β‐MCA). * P < 0.05, ** P < 0.01, and *** P < 0.001. Apc , adenomatous polyposis coli [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: CA (Aladdin, Shanghai, China), DCA (Sigma‐Aldrich, St. Louis, MO), lithocholic acid (Aladdin, Shanghai, China), chenodeoxycholic acid (CDCA; Aladdin, Shanghai, China), α‐muricholic acid (α‐MCA) (Toronto Research Chemicals, [TRC], Toronto, Canada), and β‐MCA (TRC, Toronto, Canada) were added to fecal samples as reference standards, and an external standard method was used for preliminary measurement.

Techniques: